cd3 t cell populations  (Miltenyi Biotec)

Journal: Pharmaceutics

Article Title: An mRNA-Based Multiple Antigenic Gene Expression System Delivered by Engineered Salmonella for Severe Fever with Thrombocytopenia Syndrome and Assessment of Its Immunogenicity and Protection Using a Human DC-SIGN-Transduced Mouse Model

doi: 10.3390/pharmaceutics15051339

Figure Lengend Snippet: Constructs for the SFTSV vaccine elicit cellular immune reactions. Two-week post-booster immunization splenocytes were collected and stimulated with each antigen. ( A ) At 48 h post-stimulation, changes in Th1 and Th2 cytokine profiles were assessed by qRT-PCR. ( B ) Splenocyte proliferation indices in immunized mice were compared with the control group. ( C ) Representative scatter plot diagrams of CD4 + and CD8 + sub-T cell populations were gated from a CD3 + population. ( D ) Upon stimulation with SFTSV antigens, the percentages in CD4 + and CD8 + T cell subpopulations were evaluated by flow cytometry. * p ≤ 0.05, ** p ≤ 0.001, *** p ≤ 0.005, and **** p ≤ 0.0001 indicate significant differences compared to the vector control. Error bar: mean ± S.D.

Article Snippet: Finally, cells were washed with FACS running buffer (Miltenyi Biotec), and the T-cell populations CD3 + CD4 + and CD3 + CD8 + were gated from the CD3 + population and analyzed using the MacsQuant analysis system (Miltenyi Biotec).

Techniques: Construct, Quantitative RT-PCR, Control, Flow Cytometry, Plasmid Preparation