cd3 t cell populations (Miltenyi Biotec)

Miltenyi Biotec is a verified supplier

Miltenyi Biotec manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Miltenyi Biotec cd3 t cell populations
Cd3 T Cell Populations, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cd3+t+cell+populations/CD8%2B+T+Cell+Isolation+Kit%2C+human/pm39828101-84-6-28
Average 97 stars, based on 374 article reviews

cd3 t cell populations - by Bioz Stars, 2026-10

97/100 stars

Images

Isolation:

Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy.
Article Snippet: Cell line identity was confirmed by STR fingerprinted (Eurofins, Luxemburg). .. Magnetic-activated cell separation isolated CD8+ and CD3+ T cell populations [CD8+ T cell isolation kit as previously described [10,28] or Pan T Cell Isolation Kit for CD3+ selection; Miltenyi Biotec] from human peripheral blood mononuclear cells from healthy donors (reviewed and approved by Charité ethics approval EA2/262/20). .. CD8+ L1CAM-CAR T cells (used for in vitro experiments) were generated and expanded as previously described [28] and supplied with 0.5 ng/ml IL15 (Miltenyi Biotec) and 50U/ml IL2 (Novartis).

Article Title: Targeting MYCN upregulates L1CAM tumor antigen in MYCN-dysregulated neuroblastoma to increase CAR T cell efficacy
Article Snippet: Cell line identity was confirmed by STR fingerprinted (Eurofins, Luxemburg). .. Magnetic-activated cell separation isolated CD8 + and CD3 + T cell populations [CD8 + T cell isolation kit as previously described ( , ) or Pan T Cell Isolation Kit for CD3 + selection; Miltenyi Biotec] from human peripheral blood mononuclear cells from healthy donors (Charité ethics approval EA2/262/20). .. CD8 + L1CAM-CAR T cells (used for in vitro experiments) were generated and expanded as previously described ( ) and supplied with 0.5ng/ml IL15 (Miltenyi Biotec) and 50U/ml IL2 (Novartis).

Article Title: A PD-L1-targeting chimeric switch receptor enhances efficacy of CAR-T cell for pleural and peritoneal metastasis
Article Snippet: LymphoprepTM (Axis-Shield) was used to isolate and purify peripheral blood mononuclear cells (PBMCs) by gradient centrifugation. .. Then CD3 + T cell populations were isolated by CD3 MicroBeads (Miltenyi Biotec) according to the manufacturer’s instruction, and culured in X-VIVO 15 medium, supplemented with 10% human AB serum, 10 mM N-acetyl-cysteine, 10 mM glutamine, and 10 mM MEM amino acid (all purchased from Sigma-Aldrich), as well as 100 IU/ml rhIL-2, 5 ng/ml rhIL-7, and 5 ng/ml rhIL-15 (all purchased from Novoprotein). .. After stimulation by Dynabeads TM Human T-Expander CD3/CD28 (Gibco) for 24 h, virus was added to activated T cells in RetroNectin-coated plate and centrifuged for 2 h at 1000 × g . Twenty-four hours after transfection, T cells were replaced with fresh medium.

Article Title: A PD-L1-targeting chimeric switch receptor enhances efficacy of CAR-T cell for pleural and peritoneal metastasis.
Article Snippet: LymphoprepTM (Axis-Shield) was used to isolate and purify peripheral blood mononuclear cells (PBMCs) by gradient centrifugation. .. Then CD3+ T cell populations were isolated by CD3 MicroBeads (Miltenyi Biotec) according to the manufacturer’s instruction, and culured in X-VIVO 15 medium, supplemented with 10% human AB serum, 10 mM N-acetyl-cysteine, 10mM glutamine, and 10mM MEM amino acid (all purchased from Sigma-Aldrich), as well as 100 IU/ml rhIL-2, 5 ng/ml rhIL-7, and 5 ng/ml rhIL-15 (all purchased from Novoprotein). .. After stimulation by DynabeadsTM Human T-Expander CD3/CD28 (Gibco) for 24 h, virus was added to activated T cells in RetroNectin-coated plate and centrifuged for 2 h at 1000 × g. Twenty-four hours after transfection, T cells were replaced with fresh medium.

Cell Isolation:

Selection:

Article Title: Blockade of PD-1/PD-L1 immune checkpoint during DC vaccination induces potent protective immunity against breast cancer in hu-SCID mice.
Article Snippet: 0304-3835/$ see front matter 2013 Elsevier Ireland Ltd. All rights reserved. http://dx.doi.org/10.1016/j.canlet.2013.03.010 ⇑ Corresponding authors at: Department of Immunology, Medical College of Soochow University, Suzhou, Jiangsu, China.. Tel./fax: +86 512 65125022.. E-mail addresses: songguang_ju@hotmail.com (S. Ju), xueguangzh@yahoo.com.cn (X. Zhang).

Purification:

Magnetic Cell Separation:

Activation Assay:

Article Title: Transcriptomic analysis of hepatocellular carcinoma reveals molecular features of disease progression and tumor immune biology
Article Snippet: Measurements were read using the Molecular Devices SpectraMax Microplate Reader and protein concentration was measured according to the controls and standards. .. For T cell activation, total PBMC samples from healthy donors (ALLCELLS, Alameda, CA) were enriched for CD3+ T cell populations using CD3 MicroBeads (Miltenyi Biotec, Auburn, CA, cat # 130-050-101) according to manufacturer’s instructions. .. Cells were then maintained in OpTmizer CTS T Cell expansion SFM plus T cell expansion supplement (Gibco, Grand Island, NY, cat # A1048501) complete with 5% CTS Immune Cell SR (Gibco, cat # A2596101), 2 mM Glutamine (Gibco, cat # 35050-061) and P/S solution.

Review